Journal: Bio-protocol
Article Title: A Mouse Model of Postoperative Pain
doi: 10.21769/BioProtoc.3140
Figure Lengend Snippet: The mouse was continuously anesthetized through a nose cone with 1.5%–2% isoflurane.
Article Snippet: Materials and Reagents list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Stainless steel sterile No. 11 surgical blade (World Precision Instruments, Feather Safety Razor Co. Ltd., catalog number: 504170) Sterile 5–0 nylon surgical sutures (AD Surgical, Unify, catalog number: S-N518R13) Surgical tape (3M, Transpore, catalog number: 1527–0) Cotton swab (VWR, Critical Swab, catalog number: 89031–270) Glad Press’n Seal (SAI Infusion Technologies, Glad, catalog number: PSS-70) Sterile nitrile gloves (Kimberly-Clark Professional, Kimtech Pure, catalog number: HC61170) Petri dishes [VWR, 14.5 and 9 663161, Greiner Bio-One, catalog numbers: 82050–912 (small) and 82050–600 (large)] Sterile gauze (Allied Medical, Ardes, catalog number: {"type":"entrez-nucleotide","attrs":{"text":"GA441221","term_id":"528633514","term_text":"GA441221"}} GA441221 ) Sharpie extra fine point permanent marker (Staples, Sharpie, catalog number: 37001) 8–16 week old C57BL/6J mice (JAX, catalog number: 000664) Bacitracin zinc ointment (Fougera Pharmaceuticals Inc, catalog number: 0168–0011-04) Isoflurane (Clipper distributing company LLC., Phoenix, catalog number: 0010250) 75% ethanol (Fisher Scientific, Decon Laboratories, Inc., catalog number: 22–281-562) Surgical scrub 7.5% povidone-iodine (Betadine, Veterinary, catalog number: 67618–154-01) Eye lube (Patterson Veterinary, Optixcare Ophthalmic, catalog number: 07–893-2779) Equipment list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 1,000 ml beaker (VWR, PYREX, catalog number: 13912–284) #55 Dumostar Forceps (Fine Science Tools, Dumont, catalog number: 11295–51) Scalpel handle (Fine Science Tools, catalog number: 10003–12) Iris Forceps, 10 cm, Curved, Serrated (World Precision Instruments, catalog number: 15915) Halsted Mosquito Hemostatic Forceps, 12.5 cm, Straight (World Precision Instruments, catalog number: 15920-G) Vannas Scissors, 8 cm, Curved (World Precision Instruments, catalog number: 14122) Small animal surgery board (Braintree Scientific, Inc., CD+, catalog number: ACD 014) Isoflurane dispenser (Highland Medical Equipment, Drager, catalog number: 16–7001) Sliding top isoflurane induction chamber (Kent Scientific Corporation, catalog number: VetFlo-0530LG) Heat Therapy Pump with Pad (Adroit Medical Systems, catalog number: HTP-1500) Isothermal pad (Braintree Scientific, Inc., Deltaphase, catalog number: DPIP) Digital calipers (VWR, catalog number: 62379–531) Steri 250 Bead Sterilizer Bead Bath (Lab Unlimited, Simon Keller Ltd., catalog number: 4AJ-6286283) Microwave (Emerson, 1,000 W, catalog number: B007Q45CIS) Home cage containing Aspen Sani Chips® (P.J.
Techniques:
